This article discusses how A-TEEM molecular fingerprinting can be used for pharmaceutical analysis. It will highlight exciting results in the field of wine and spirits phenolics, pharmaceutical analysis, olive oil adulteration, and water and trace PAH oil analysis using HORIBA’s A-TEEM Molecular Fingerprinting technique.
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Horiba开发了专利的专用仪器和软件方法，主要关注纳米技术和水处理监测应用。最重要的发展包括利用多通道检测器的同时吸收荧光仪器，这些探测器扩大了传统的荧光激发发射矩阵以外的能力。
Classic Fluorescence Measurements
经典的荧光测量通常涉及激发和发射光谱，传统上是单独制造的。
通常将荧光强度作为发射光谱或激发或吸收波长或激发光谱的发射波长表示。这对于样品中有一个物种的样品非常有效。但是，如果有许多不同的物种，例如多组分混合物和复合物，则该物种也不起作用，因为许多不同类型的化学品具有光谱重叠。该附加变量与组件的其他相对浓度和绝对浓度有关。
常规溶液是激发发射矩阵（EEM），也称为3D荧光或总荧光扫描。这将激发发射矩阵分开，将其融合到一个3D轮廓图中。
常规仪器将在给定的发射波长下进行激发扫描，并提供有关样品中不同组件数量的视觉定性信息，这主要是因为样品中的每种其他化学物质都具有独特的吸收激发和发射波长坐标形状。
在过去的几十年中，涉及这种EEM类型的测量的出版物逐渐增加。
申请of Excitation-emission Matrix Techniques
There are many different types of EEM applications, including studies of the environment, water quality, and toxicity effects.
EEM已成为各个研究领域中广泛接受的工具。
A problem with EEMs is a phenomenon known as Inner Filter Effects.
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The primary inner filter effect of the excitation beam path, and the secondary absorption of the emission beam path work against fluorescence measurements with this EEM technology, which means that users have to work with very dilute solutions.
Horiba的A-Teem技术的主要好处是同时测量吸收和荧光性能，从而纠正了这种内部过滤器效应。这有助于构建复合库和多元统计模型。
Horiba的Aqualog
Aqualog是Horiba释放的第一个A-TEEM光学仪器，并且是UV-VIS吸收系统的真正杂交。
One of the instrument's key features is its charge-coupled device (CCD) detector, which greatly increases the acquisition speed.
Previous implementations of EEM, where they had inner filter effects, required separate instruments for UV-Vis and fluorescence measurements. On top of that, there is a scanning system in the emission path, much like we have in the absorption path. The Aqualog system gives much faster scanning and true simultaneous absorption and fluorescence excitation-emission mapping.
A-TEEM允许吸光度测量，可提供有关所有可能不荧光的有色物种的信息。
A-TEEMprovides absorption information for the sample, providing data on the colors that may be weakly fluorescent or non-fluorescent.
用户还可以计算透射率，各种颜色和色度指数，同时接收吸收信息是有益的。
区分不同的分子并制作模型来量化不同的分子
Aqualog can measure and provide linear responses for the fluorescence information up to the Beer-Lambert linear relationship. This determines the linear relation between absorbance and concentration for any chemical and solution. This expands a linear concentration range for fluorescence, provides very high sensitivity, allows users to resolve low-concentration samples from high-concentration backgrounds, and produces effective libraries in multi-various statistical models that enable users to identify compounds over wide concentration ranges.
The Aqualog collects data much faster than trying to scan each excitation and emission wavelength in serial mode. With the HORIBA A-TEEM system, users can measure both colored and fluorescent compounds as well as scattering compounds simultaneously. They can also carry out CIE chromaticity and CIELAB measurements.
Aqualog can correct the inner filter effects as data is collected. Data can be applied and formatted to carry out the multi-block organization of absorbance and fluorescence, significantly enhancing the statistical significance of many multivariate statistical models.
HORIBA’s new FAST-01 Autosampler allows batch sampling with a fully temperature-controlled sample changer. There is an insulated sample chamber that can deal with several different types of vial and tray applications.
水消毒
The Aqualog was originally developed for drinking water and disinfection. The primary goal of a drinking water treatment plant is to disinfect the water.
微生物污染引起了历史上的许多疾病。来自任何天然河流或水库的水都包含几种自然溶解的芳香材料或天然有机物。欧洲杯足球竞彩这种无处不在的混合物来自从流域中浸出土壤中的化合物，进入饮用水处理厂的来源。
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Chlorine reacts with this and leads to toxic disinfection byproducts associated with various types of carcinogenic activity and various reproductive issues.
Horiba的AutoSampler允许用户从几乎任何感兴趣的来源中在饮用水处理厂中取样。Aqualog是插头流动池操作。
超过1000万人受到健康水平的关注，在美国，全国一千多个系统。使用Aqualog，用户可以不断监视溶解的有机碳，并量化和限定有机物的不同组成部分，以照顾消毒副产品规则。
该平台还可以监视与石油溢出有关的藻类和油PAH和BTEX。
Assessing Wine Quality with the A-TEEM
本节将讨论有关酚类和花青素的HPLC级特异性的三个应用程序。
Aqualog可以评估碱性化学，包括pH，酒精和亚硫酸盐。
有了这个系统，我们有一站式葡萄酒化学商店。霍里巴（Horiba）的一些葡萄酒客户还使用它来评估该葡萄酒是否被烟熏味。
除了识别腐败化合物外，用户还可以识别用于使葡萄酒深红色和橡木桶提取化合物的颜色添加剂，以处理风味和口感问题。
A-TEEM是一项非常广泛的技术，提供了几种协同的交互。这样，吸收和传输数据，颜色信息和色调强度可用于定性和质量控制的名称。
图片来源：Shutterstock/Andrey Bayda
EEM指纹识别特定的组件，这些组件将与葡萄酒质量有关的更具体的信息（例如，洛特和区域品种过程和发育特征）。
Users can apply regression and multi-block analyses to calibrate the EEM information.
This can be used to evaluate and calibrate against reference chemistry techniques such as reverse-phase HPLC, gas and liquid chromatography, mass spectroscopy, and the ability to calibrate with pure chemical standards or mixtures as a primary method.
主要目标是拥有有关这些化学类型的非常具体的信息。通过结合吸收光谱和荧光EEM光谱的变量来评估的许多最有效的模型为用户提供了来自弱荧光和高度荧光化学物质的杠杆和信息。
尽管每种化学物质都有其自身的吸收和发射光谱，但被称为量子产率的吸收和发射光谱之间存在独特的关系。浓度和称为灭绝系数的吸收之间也存在独特的关系。
The absorption and fluorescence data greatly increase the ability to distinguish different chemicals based on the extinction fluorescence quantum yield and spectral shapes. Therefore, the concentration, sensitivity, and selectivity can be taken advantage of and further derivations of the absorption data can be considered.
EEM technology makes molecular- and isomeric-specific designations of different compounds. Statistically, the Parallel Factor Analysis can be applied using principle components assessments that identify absorption and emission spectral shapes as loadings and concentrations as scores.
还有一个即时色谱积分器和报告生成器，称为Horiba多模型应用程序工具。
A-TEEM着眼于不同的酚类，在多模型应用程序工具的框架内验证和验证这些特定模型化合物中的每一个。用户可以鉴定不同物种和品种的葡萄以及在发酵过程中如何反应葡萄。
Identifying Olive Oil
Being able to identify extra virgin olive oil is very important.
图片来源：Shutterstock/Luigi Giordano
HORIBA’s platform can differentiate between different types of olive oils, including extra virgin and machine-processed, and determine extra light fractions by evaluating their chlorophyll and pheophytin content, oxidation and degradation products, compound, and component two, which is the oxidation product.
Users can identify olive oil freshness using this information. The breakdown of and the loss of magnesium and chlorophyll is a good indicator of olive oil freshness.
Differentiating Cannabinoids
大麻花主要包括酸性或无活性的羧化形式，例如THC和CBDA。
研究人员可以主要根据A-TEEM数据区分这些不同的分子，并可以测量CBD与THC比率。
Horiba的解决方案还有助于确定不同花和与大麻素相关的各种纯化过程类型的脱羧和激活水平。
The Aqualog and A-TEEM is roughly a hundred-fold more sensitive in terms of the absolute plant material compared to conventional methods.
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药物应用
The main objective of this application is to validate that the vaccines are in the proper packaging at the final stage. This replaces a potency test that normally takes up to 30 days.
Researchers can take a simple A-TEEM spectral fingerprint by simply solubilizing the vaccine, conducting a rapid analysis, and identifying the vaccines in less than 30 minutes.
HORIBA offers completeness traceability for absorption and fluorescence, IOQ support, and a 21 CFR-compliant software module that enables FDA approval.
该平台还测量细胞介质，取代非常昂贵且耗时的标准色谱或质谱测量。
The A-TEEM offers a rapid holistic method that can be used for early-stage identification and material quality assessment. It can distinguish between various vendors with a calibration validation set using a multi-block technique with the extreme gradient boost method at 100% accuracy.
Users can make conclusions about age- and light-dependent interactions. They can also identify different cell media recipes with theA-Teem技术。
HORIBA’s technology and services can receive comprehensive molecular fingerprints in seconds to minutes. It is column-free and solvent-free with minimum sample preparation, while it is very easy to facilitate automated analysis and dashboards with the multi-model application tool.
此信息已从Horiba Scientific提供的材料中采购，审查和改编。欧洲杯足球竞彩
For more information on this source, please visitHoriba Scientific。