图片来源：Andor Technology
牛津仪器公司兼科学成像和光谱解决方案的世界领导者Andor Technology（Andor）今天宣布推出新的超分辨率显微镜技术，可在单个光子敏感的IXON EMCCD摄像机上使用。“ SRRF-stream”使用低照明强度下的常规荧光团在大多数现代显微镜上解锁了实时超级分辨率荧光显微镜，从而使其与活细胞成像高度兼容。对于大多数数据集，预期的分辨率从2-倍（50-150nm最终分辨率）的分辨率得到改进。
Adopting the recently developed SRRF (pronounced ‘Surf’) technology from the lab of Dr Ricardo Henriques, Quantitative Imaging and Nanobiophysics Group, University College London (UCL), and working in close collaboration with Dr Henriques, Andor have enhanced the technology to run optimally on iXon EMCCD cameras. Andor is also expert in advanced GPU processing optimization techniques, employed in this instance to execute the SRRF algorithm up to 30x faster than the existing ImageJ-based post processing implementation of SRRF. This significant acceleration enables workflow enhancement, by allowing data acquisition and SRRF processing to operate in parallel. Since processing is faster than the camera can acquire data, SRRF-Stream now accomplishes real time super-resolution, with large field of view super-resolution images. An EMCCD full field of view super-resolved image can be readily generated at a rate of > 1 Hz, easily pushing up to > 10 Hz through use of smaller ROI sizes. Regarding image acquisition software, SRRF-Stream is readily available to SDK users and has also been made compatible with the MicroManager (Open Imaging) open source microscopy software platform.
Andor的成像产品经理Colin Coates博士说；“ Andor对Henriques Lab具有共同的愿景，该实验室的愿景是使所有人都可以广泛且易于访问的超分辨率显微镜。SRRF-stream在该方向上取得了显着飞跃，与标准广场，TIRF和旋转磁盘共聚焦模式非常好。SRRF-Stream和Andor最近发布的IXON Life EMCCD的组合是一种高度成本有效的方法，可以从现有的常规荧光显微镜中解锁强大的超分辨率。”
我们很高兴与Andor合作，参加SRRF-Stream开发。由于它可以实现出色的分辨率，而无需专门的照片开关荧光团，并且比基于标准的基于基于标准的基于基于的基于标准的基于基于的基于标准的位置的超级分辨率的标准的超级分辨率技术所要求的光水平低约106倍，因此我们收到了有关SRRF的很多非常积极的评论。。我们很高兴看到SRRF-Stream实时运行，而不是后处理。在我们自己的实验室中对SRRF-Stream进行了彻底测试，我们对工作流程以及现在利用较大的视野进行实时细胞超分辨率的能力给我们留下了深刻的印象。通过无缝将SRRF算法与IXON的高性能相结合，我们完成了世界上第一个用于荧光显微镜的超分辨率摄像头。
Ricardo Henriques博士，定量成像和纳米生物物理小组，伦敦大学学院