Postnova Analytics发表了一份信息丰富的新文章,详细介绍了多探测器不对称场流分馏(AF4)和电气AF4(EAF4)技术如何改变病毒和抗体的分离和表征。
病毒是2020年的科学故事,全球冠状病毒(Covid-19)大流行,将它们刺穿了几乎每个人在世界各地的意识的最前沿。开发特定病毒的抗体构成了未来免疫反应的核心。病毒的表征,疫苗的发育和抗体的定量已成为许多领先实验室的最重要的科学追求。
疫苗可能很复杂,并且可能包含许多跨越大尺寸的物种,范围从抗体,蛋白质和核酸,多糖蛋白质复合物等病毒片段,直径通常超过100 nm。这意味着此类样本在使用基于柱的色谱技术进行分离和表征表征方面具有挑战性。新的白皮书解释并说明了AF4技术如何帮助该领域的研究人员,因为它是一种无固定相的分离技术,用于表征从大约1纳米到1微米的多分散样品的表征。AF4技术已经被越来越多的论文引用,重点是病毒和病毒样颗粒的表征以及抗体,抗原及其聚集体和聚集体。欧洲杯猜球平台
白皮书使用多探测器AF4和EAF4提出了三种应用:与腺相关病毒(AAV)单体及其片段或聚集体的分离和表征;暴露于热应激和单克隆抗体(MAB)的分离和MAB聚集体的分离后,两种AAV血清型的聚集。
The authors of this new white paper conclude that the open channel design of Postnova Analytics’ AF4 system has advantages over chromatographic techniques, as the absence of a stationary phase results in less sample loss due to interaction with, or filtration by, the column packing material. Further, from the data presented, it can be seen that AF4 can separate a wide range of sizes using a single method, from virus fragments less than 10 nm in radius, to large aggregates above 100 nm in radius. The powerful yet versatile AF4 technique can be equally applied to any or all the components in virus and vaccine research from small antibodies of only a few nm in size, up to large protein-polysaccharide complexes or virus particles and aggregates of well over 100 nm.
有关新纸的副本,请访问https://www.postnova.com/news-and-events/press-releases/256-white-paper-separation-separation-and-characterization-of-viruses-viruses-viruses-and-antibodies.html。