In an article recently published in the journalActa Biomaterialia，研究人员讨论了抗菌，粘性和自我修复水凝胶的准备，以治愈糖尿病伤口。
学习：Antibacterial adhesive self-healing hydrogels to promote diabetic wound healing。图片来源：十亿照片/shutterstock.com
背景
长期糖尿病会损害伤口愈合，导致慢性非愈合伤口和其他并发症。糖尿病伤口疾病的治疗期很长，由于其复杂性，医疗费用相当大。迫切需要有效的治疗技术，以确保患者减轻疼痛，增加生活质量以及伤口愈合的超速。
Wound dressings, such as hydrogel dressings, transparent film dressings, liquid dressings, and foam dressings are designed and applied to aid in the healing of injured wounds.
水凝胶伤口敷料是伤口护理中最有前途的材料之一。欧洲杯足球竞彩明胶是一种非常具有成本效益的基本材料，用于制作糖尿病伤口水凝胶敷料。腺嘌呤可以通过形成氢键和金属络合物与材料成分，因为其独特的分子结构。
铜2+doping in gelatin/adenine-derived hydrogels not only ensures the hydrogel's integrity to prevent wound infection but also endows the hydrogel with angiogenic and antibacterial capabilities, which could aid diabetic wound healing. Such multi-functional hydrogels, however, have yet to be discovered.
关于研究
在这项研究中，作者讨论了基于丙烯酸丙烯酸酯（AA），明胶甲基丙烯酸酯（GELMA）和CUCL的各种粘性，自我修复和抗菌水凝胶的发展。2通过CU的配位络合来促进糖尿病伤口愈合2+和羧基，共价键和氢键。
该团队使用氢键，自由基聚合和离子键合作设计和准备各种粘合剂，自我修复和抗菌水凝胶。通过自由基聚合，明胶通过甲基丙烯酸酐改变，以产生具有改善稳定性和机械特性的水凝胶。自由基聚合用丙烯酰氯化后，使用自由基聚合将腺嘌呤掺入水凝胶网络中。铜2+还通过Cu的配位引入了Gelma/AA水凝胶中2+with the carboxyl groups of gelatin, which resulted in the development of GelMA/AA/Cu hydrogels.
The researchers tested the developed hydrogels for self-healing, mechanical properties, Cu2+release, antibacterial capabilities, biocompatibility, and hemostasis in vivo. Furthermore, diabetic mice with full-thickness skin defects were created, and the GelMA/AA/Cu hydrogel's effect on diabetic wound healing was assessed.
观察
孵育12小时后，S.金黄色葡萄酒生存率为11.1％，而大肠杆菌完全被压制。孵育3小时后，gelma/aa/cu1.0组中近一半的细菌被杀死，细菌存活率的比率S.金黄色葡萄酒和大肠杆菌were both around 50.0%. The survival ratios ofS.金黄色葡萄酒和大肠杆菌孵育9小时后分别为7.5％和0.0％。此外，GELMA/AA/CU1.0水凝胶可能会在大约12小时内完全破坏细菌。孵育3小时后，水凝胶凝胶/AA/CU1.5组完全阻止S.金黄色葡萄酒和大肠杆菌。
随着Cu的增加2+浓度，GELMA/AA/CU水凝胶的粘合强度从3.4 0.4 kPa增加到9.3 0.1 kPa。在对照组中，失血为224.0±39.9 mg，而在Gelma/AA/CU1.0水凝胶组中，失血量为23.0±31.9 mg。GELMA组，对照组，Gelma/AA/CU1.0组和Gelma/AA/CU0组的胶原蛋白含量分别为110.1±14.3％，99.9±18.0％，160.1±14.9％和108.4±14.4±14.4±14.4±14.4±14.4±14.4±14.4±14.5％。
Due to the metal-ligand interaction and hydrogen bond provided by the Cu2+和the carboxyl group, the developed hydrogels had excellent fatigue resistance, rapid self-healing characteristics, and good adhesion qualities. In a mouse liver trauma model, the GelMA/AA/Cu1.0 hydrogel comprised of 1.0 mg/mL Cu2+具有良好平衡的抗菌和生物相容性特性表现出出色的止血性能，并且在全厚性皮肤糖尿病伤口模型中大大辅助伤口愈合。
When compared to the TegadermTM值薄膜，Gelma和Gelma/AA/Cu0水凝胶的免疫组织化学结果表明，Gelma/AA/CU1.0水凝胶可以促进正常的胶原沉积和上皮化。根据免疫荧光数据，GELMA/AA/CU1.0水凝胶可以降低促炎因子的表达并增加血管生成。
结论
总之，这项研究阐明了制备的粘合剂，自我修复和抗菌凝胶/AA/CU水凝胶伤口敷料被证明成功地促进了糖尿病皮肤伤口的再生。
Hydrogen bonding and metal-ligand coordination provided outstanding self-healing characteristics. In comparison to the TCP group, GelMA/AA/Cu hydrogels demonstrated good biocompatibility in the L929 cell cytocompatibility test. The inclusion of Cu2+facilitated the GelMA/AA/Cu hydrogel’s remarkable antibacterial characteristics, which helped to prevent infection in diabetic wounds. The GelMA/AA/Cu hydrogel also performed well in terms of hemostasis. Furthermore, the GelMA/AA/Cu1.0 hydrogel's superior wound healing impact on diabetic wounds was demonstrated by the downregulation of IL-6 and the acceleration of vascular regeneration in wound healing (α-SMA and CD31).
作者强调，Gelma/AA/CU水凝胶是糖尿病伤口愈合的有前途的材料。欧洲杯足球竞彩
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来源
Chen, J., He, J., Yang, Y., et al. Antibacterial adhesive self-healing hydrogels to promote diabetic wound healing. Acta Biomateriaia (2022).https://www.欧洲杯线上买球sciendirect.com/science/article/abs/pii/s1742706122002501
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