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How Can We Bioprint Plant Cells?

A new paper published in生物打印已经回顾了3D植物细胞生物打印的领域。当前和未来的观点已在审查研究中得到了彻底的研究,以及当前技术和潜在的未来应用的局限性。来自法国Segula Technologies和LyonUniversité大学的科学家为该论文做出了贡献。

学习:植物细胞的绿色3D生物打印:3D生物打印的新范围。图片来源:luchschenf/shutterstock.com

生物打印

添加剂制造(也称为3D打印)已在多个行业中广泛应用,以从陶瓷,金属,聚合物和生物材料等材料中生产许多商业产品。欧洲杯足球竞彩这些制造工艺比传统方法提供了重大好处,包括自由形式设计,多物质印刷以及减少的废物和后处理步骤。欧洲杯足球竞彩

Additive manufacturing has shown promise in the field of biofabrication. Alongside the development of additive manufacturing processes, progress in tissue engineering has allowed the production of biomaterials with complex architectures and functional properties using additive manufacturing processes. This provides significant benefits for regenerative medicine.

Tissue constructs are produced using bioink, which can be produced from a multitude of biomaterials and living cells. Bioink selection is crucial to fabricating constructs with the desired shape and properties, as well as allowing the growth and proliferation of viable cells within the three dimensional engineered tissue architecture after printing. Polymer hydrogels are commonly used for this purpose.

Different 3D bioprinting methods have been developed in recent years, including inkjet bioprinting and microextrusion bioprinting. Microextrusion bioprinting offers the possibility of highly populated, centimeter-scale scaffolds which are similar in size and shape to organic structures. Moreover, viscous hydrogels can be printed using microextrusion techniques.

近年来开发的其他方法包括使用激光辅助生物打印的基于光的方法和单细胞制造工艺。其中一些方法的成功有限。3D植物细胞生物打印的领域对食品和农业产业以及生物电力产生等领域有好处,已获得了增加的研究重点。

研究

新评论研究了3D绿色植物细胞生物打印中最新的最新作品。作者已经强调并研究了一些前保期研究,以提供有关绿色植物细胞组织印刷的当前观点。综述已经分析了66项研究。

作者注意到缺乏研究,从组织工程的进步中获得的知识并将其应用于植物组织培养物以产生功能性组织。目前,绿色植物细胞生物打印处于起步阶段。

最近关于绿色生物打印的研究

自2015年引入绿色生物打印以来,该领域已经进行了几项研究。食品工业已经印刷了可食用的植物细胞,目的是达到与天然食品类似的质地。例如,生菜叶细胞已用于筛选基于果胶的生物墨水组成。

Another recent study ofZinnia elegans,通过修饰3D印刷构建体中的植物激素来证明细胞分化。这项研究为3D打印植物细胞结构特性的研究和控制提供了相关信息。

已经开发出光辅助方法是在光合链接水凝胶中间接打印水稻细胞。研究已经印刷了具有增强的代谢活性的水稻细胞,可用于分泌生物脱位剂对神经剂,神经生成疾病和可卡因毒性的分泌。

作者指出,目前的研究主要集中在土地植物细胞上,几乎没有研究微藻类。单个和聚类的细胞已用于研究,一项研究报告了一百个细胞的亲聚体的生物打印,这需要大量的生物构图。尽管该领域仍处于起步阶段,但有关植物细胞生物打印的研究数量正在增长。

生物学和后处理方法

Bioink formulation impacts the printing of plant cells in the same way as animal cells. Common bioinks used for plant cell bioprinting include methylcellulose, gellan cum, nanocellulose, carrageenan, agarose, bacterial cellulose, alginate, silk, and pectin. Most of the current studies have reported the use of agarose, but on its own, this biomaterial suffers from shear-thinning behaviors, facilitating the use of other biomaterials.

近年来,还制定了几种后处理策略,以提高印刷产品中的可扩展性和形状稳定性。已经评估了添加剂和交联剂的改善尺寸稳定性以及物理和机械性能。正在进行研究,以改善后处理步骤,以产生可行的3D打印植物细胞组织。

总之

The review has comprehensively explored the field of 3D printed plant cells, highlighting several studies in the current literature. Current advances in the field are promising, but challenges still exist that need to be addressed by researchers.

本文中强调的将来的观点是可以通过调节细胞的微环境来控制细胞命运。这为实现植物器官发生并生产木材等专业植物组织提供了可能性,并提供了生产可再生资源的新途径。实验室生产的食品也有很大的机会。

More from AZoM: Use and Applications of Flame Atomic Absorption Spectroscopy

进一步阅读

Landerneau, S et al. (2022) Green 3D bioprinting of plant cells: A new scope for 3D bioprinting生物打印e00216 [在线,预隔离] ScienceDire欧洲杯线上买球ct.com。可用网址:https://www.sciencedirect.com/science/article/pii/S2405886622000264?via%3Dihub

Disclaimer: The views expressed here are those of the author expressed in their private capacity and do not necessarily represent the views of AZoM.com Limited T/A AZoNetwork the owner and operator of this website. This disclaimer forms part of theTerms and conditionsof use of this website.

雷金纳德·戴维(Reginald Davey)

写的

雷金纳德·戴维(Reginald Davey)

Reg Davey是英国诺丁汉的自由撰稿人和编辑。为新闻医学写作代表了他多年来一直感兴趣并参与的各种兴趣和领域的融合,包括微生物学,生物医学科学和环境科学。欧洲杯线上买球

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